Methodological Challenges of Digital PCR Detection of the Histone H3 K27M Somatic Variant in Cerebrospinal Fluid

Cell-free DNA (cfDNA) in body fluids is invaluable for cancer diagnostics.Despite the impressive potential of liquid biopsies for the diagnostics of central nervous system (CNS) tumors, a number of challenges prevent introducing this approach into routine laboratory practice.In this study, we adopt a protocol for sensitive detection of the H3 K27M

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Measuring Genetic Robustness in Vesicular Stomatitis Virus

Genetic robustness is the ability of a genome to incorporate mutations with the result of no fitness changes.Thus, more robust viruses have an increased neutral mutation rate.This property is Liquor Bags particularly important in RNA viruses due to their high mutation rates.The most direct way of measuring robustness in vesicular stomatitis virus (

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